GC MS – Analysis of Bio Active Compounds from Propolis and Antibacterial Activity against B.cereus Isolated from Tasar Silkworm Cadavers

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1Gudimalla Prashanthi and 2Dr. Kuntamalla Sujatha*

Bulletin of Pure and Applied Sciences.

Botany, Vol.42 B, No.2.
July-December 2023: P.65-72

Original Research Article

Description

Description

1Gudimalla Prashanthi and 2Dr. Kuntamalla Sujatha*

Author’s Affiliation

1Research Scholar, Department of Sericulture, Kakatiya University, Warangal, Telangana 506009, India

E-mail: prashanthigudimalla@gmail.com

2Assistant Professor, Department of Sericulture, Kakatiya University, Warangal, Telangana 506009, India

E-mail: k.sujatha8900@gmail.com

*Corresponding Author:

Dr. Kuntamalla Sujatha

Assistant Professor, Department of Sericulture,

Kakatiya University, Warangal,

Telangana 506009, India

E-mail:

k.sujatha8900@gmail.com

 

Received on 13.08.2023

Revised on 23.10.2023

Approved on 22.11.2023

Accepted on 29.11.2023

Published on 23.12.2023

Abstract
Propolis is a sticky, non toxic, soft resinous substance produced by honey bees. The chemical composition of propolis depends upon the collecting time, location and plant source resulting in the biological activity variation. The aim of this study was to analyze the chemical composition of propolis by using Gas chromatography – Mass spectrometer. Bee propolis is a natural medication applied topically and is considered to have antibacterial, antifungal, antiviral, antibiotic, antioxidant properties and is used for treatment of many infections. Propolis was found to be rich in alkaloids, saponins, tannins and resins. The chemical characterization revealed the presence of 9 distinct phytochemical compounds using GC – MS and the most predominant compound were S Methyl – L – Cysteine (62.36%) followed by Cis – 2,3 – Epoxyoctane (31.78%). The different concentrations of methanolic extracts of propolis showed varied antibacterial activities against B. cereus with highest zone of inhibition (19.0 mm).