Electrophoretic Banding Patterns of Esterase Isozymes in Fresh Water Fish Channa punctatus
15.63$
Year : 2022,
Volume & Issue : BPAS-Zoology 41A(1), JAN-JUN 2022
Page No. : 34-39,
Article Type : Original Aticle
Article DOI : 10.5958/2320-3188.2022.00005.5
Article Info: Received on 26.07.2021 Revised on 19.12.2021 Accepted on 15.01.2022 Published on 15.06.2022
Description
Description
Dr. M. Venkateswara Rao1, *Dr. Y. Venkaiah2
Author’s Affiliation : 1Assistant Professor, Dept. of Zoology, SR & BGNR Degree College, Khammam, Telangana 507002, India.
E-mail: venkatmandalapu18@gmail.com, 2Associate Professor of Zoology, Department of Zoology, Kakatiya University, Warangal, Telangana State 506009, India
E-mail: venkaiahyanamala07@gmail.com
Corresponding Author : Dr. Y. Venkaiah Associate Professor of Zoology, Department of Zoology, Kakatiya University, Warangal, Telangana State 506009, India,
E-Mail:-venkaiahyanamala07@gmail.com
Abstract
Esterase enzymes catalyse the formation and breakdown of carboxylic acid esters of alcohols. The present work aimed to study the electrophoretic banding patterns of esterase isozymes in fresh water fish Channa punctatus The results revealed that the electrophoretic esterase banding patterns varied in different tissues i.e. gill, liver, intestine, muscle, and brain of fish c. puctatus Esterase patterns were separated on thin layer 1.5mm (thickness) polyacrylamide gels (SDS-7.5٪) and stained with α-naphthyl acetate used as substrate. Three different esterase bands were detected and named as Esi-1; Est-2; and Est-3; with different relative mobilities such as 0.6 ±0.05; 0.4±0.05; 0.3±0.05. All the three esterase bands were found in all tissues i.e. gill, liver, intestine, muscle and brain. Among the three esterases Est-1 is found in all the tissues. Est-2and Est-3 were found in all the tissues. Studies on esterases of fishes and other organisms revealed similar type of patterns of esterase were noticed in one or the other tissue of all the animals.
How to cite this article: Rao MV, Venkaiah Y. (2022). Electrophoretic Banding Patterns of Esterase Isozymes in Fresh Water Fish Channa punctatus. Bulletin of Pure and Applied Sciences-Zoology, 41A (1), 34-39.
Keywords α-naphthylacetate, Channa punctatus, Electrophoretic banding patterns, Esterase.
How to cite this article: Rao MV, Venkaiah Y. (2022). Electrophoretic Banding Patterns of Esterase Isozymes in Fresh Water Fish Channa punctatus. Bulletin of Pure and Applied Sciences-Zoology, 41A (1), 34-39.
Keywords α-naphthylacetate, Channa punctatus, Electrophoretic banding patterns, Esterase.
