Evaluation of Antimicrobial Activity of Brassica juncea Leaves against Different Strains of Bacteria
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Year : 2022,
Volume & Issue : BIO SCI 38(1), JAN-JUN 2022
Page No. : 26-34,
Article Type : Original Aticle
Article DOI : 10.5958/2320-3161.2022.00003.7 (Received on 30.09.2021 Revised on 29.01.2022 Accepted on 04.02.2022)
Description
Description
1Surbhi Verma, 2Brahm Kumar Tiwari*, 3Nidhi Jaiswal, 4Fanish Kumar Pandey
Author’s Affiliation : 1Department of Biotechnology, I.T.S Institute of Health and Allied Sciences, Muradnagar, Ghaziabad, Uttar Pradesh 201206, India. 2Faculty of Allied Health Sciences, SGT University, Gurugram, Haryana 122505, India 3Department of food and Nutrition, Lady Irwin College, University of Delhi, Delhi 110001, India. 4Department of Biotechnology, Institute of Applied Medicine and Research, Ghaziabad, Uttar Pradesh 201206, India.
Corresponding Author : Faculty of Allied Health Sciences, SGT University, Gurugram, Haryana 122505, India ,
E-Mail:-brahmbiochem@gmail.com
Abstract
A large number of plants possess medicinal properties due to which they are used as therapeutic agents or an active ingredient of a medicinal preparation. A sizeable fraction of the population throughout the world depends on traditional practices to fight against various infections and diseases and nearly all of it involves the use of plant extracts gathered from a vast diversity of plants which may include Herbs, Shrubs, Trees, Climbers or Creepers. In the present work, we have attempted to study different characteristics of Brassica juncea leaves in different solvents including ethanol, methanol and distilled water, to understand its antimicrobial and antioxidant activity. Brassica juncea comes under the family Cruciferae and genus Brassica, is an important Rabi season oilseed crop. Various tests were carried out to estimate phytochemicals like tannins, flavonoids, phenols, alkaloids, glycosides, terpenoids, amino acids, steroids, anthroquinones, and saponins. Methanol extract of plant leaves were came out to be abundant in phenols and flavonoids. The antioxidant activity of B. juncea was determined by DPPH scavenging activity and it is revealed that methanol extract of this plant leaves possess maximum antioxidant activity. Antibacterial potential of plant leaves extracts were determined in vitro by the help of agar well diffusion method against Staphylococcus aureus, Bacillus subtilis, Klebsiella pneumonia and Escherichia coli. Results were also compared with control (Piperacillin/Tazobactam). All the extracts of B. juncea leaves showed appreciable antibacterial activity.
How to cite this article: Verma S, Tiwari BK, Jaiswal N, Pandey FK. (2022). Evaluation of Antimicrobial Activity of Brassica juncea Leaves against Different Strains of Bacteria. Bio-Science Research Bulletin, 38(1), 26-34.
Keywords Antimicrobial activity, Antioxidant activity, Brassica juncea, Extracts, Phytochemicals
How to cite this article: Verma S, Tiwari BK, Jaiswal N, Pandey FK. (2022). Evaluation of Antimicrobial Activity of Brassica juncea Leaves against Different Strains of Bacteria. Bio-Science Research Bulletin, 38(1), 26-34.
Keywords Antimicrobial activity, Antioxidant activity, Brassica juncea, Extracts, Phytochemicals
