Green and Benign Strategies for Synthesis of Biologically Active Compounds using Lipase as Biocatalyst

ABSTRACT An efficient, feasible, mild, affordable, and environmentally friendly method has been developed for the synthesis of biologically active compound 3-methylene-indoline-2-one from Isatin and active methylene compounds using Lipase as a Green and Biocatalyst. Biological Processes are very important to optimization to increase and improve the efficiency of cost. In this project, Malononitrile, or Dimedone reacts with Isatin in presence of lipase. The essential features of this approach are metal-free reaction, high yield, nontoxic, and less hazardous. These reactions surpass metal-catalyzed processes because these reactions have violent reaction conditions, toxic, less yields, complex workups, and environmentally hazardous ingredients. This approach replaces hazardous chemicals with benign material during their design, manufacture, use and disposal. Designing of product is cost-effective and environmentally benign chemically products. It is completely followed to Green Approach. The entire given biologically active compound have shown biological activities such as Antiviral, Anti-HIV, Antibacterial, and Anticonvulsants. Keywords: Green Approach, Lipase, Biocatalyst, Biologically Active